BioFlex® Culture Plate
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Flexible-bottomed culture plate that works with the Flexcell® Tension System to provide a mechanical load regimen to cells in monolayer (see Fig. 1 below).
*5% Savings (based on buying 40 plates at the individual price)

Applications of BioFlex® Culture Plates
  • Mechanical Load - use with the FX-6000™ Tension System to research the effects of equibiaxial tensile strain on cells in monolayer culture.
  • Softer Substrate - provides a softer substrate for cell growth (when compared to standard plastic culture dishes) which better mimics in situ conditions for some cell types like trabecular meshwork cells (see Aga et al. Invest Ophthalmol Vis Sci 55(9):5497-509, 2014).
  • Matrix Coated Surface - culture plate surfaces are coated with various matrix proteins, such as type I collagen and laminin, to better simulate in vivo conditions (see Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment).
  • Traumatic Injury - can be used in studies looking at traumatic injury in astrocytes and neurons, where the injury is produced with a Cell Injury Controller that controls a burst of gas which deforms the BioFlex® membrane creating large biaxial strains (see Ellis et al. J Neurotrauma 12 325–339, 1995; Ahmed et al. J Neurochem 74(5):1951-1960, 2000; Augustine et al. Neuroscience 274:1-10, 2014).
Read more about Applying Mechanical Load to Cells in Monolayer Culture

Equibiaxial Strain

Figure 1: Equibiaxial strain application (with a FX-6000™ Tension System) to cells plated in the well of a BioFlex® culture plate.

Relevant Tech Reports & Other Information
Tension System Video

Tension BioFlex® Baseplate Assembly
This video details how to set-up a BioFlex® baseplate with Loading Stations™ and 6-well culture plates for use with a FX5K™ Tension FlexLink®.
.wmv / .mp4 (SD) / .mp4 (HD)

Recent Publications with a Flexcell® Tension Product

Diabetes impairs arterio-venous specification in engineered vascular tissues in a perivascular cell recruitment-dependent manner
Altalhi W, Sun X, Sivak JM, Husain M, Nunes SS. Biomaterials 119:23-32, 2017. doi: 10.1016/j.biomaterials.2016.12.003.

Blood pressure-associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding
Bai X, Mangum KD, Dee RA, Stouffer GA, Lee CR, Oni-Orisan A, Patterson C, Schisler JC, Viera AJ, Taylor JM, Mack CP. J Clin Invest 127(2):670-680, 2017. doi: 10.1172/JCI88899.

Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor
Freeman SA, Christian S, Austin P, Iu I, Graves ML, Huang L, Tang S, Coombs D, Gold MR, Roskelley CD. J Cell Sci 130(1):152-163, 2017. doi: 10.1242/jcs.180612.

Gold nanoparticle interactions with endothelial cells cultured under physiological conditions
Freese C, Anspach L, Deller RC, Richards SJ, Gibson MI, Kirkpatrick CJ, Unger RE. Biomater Sci 5(4):707-717, 2017. doi: 10.1039/c6bm00853d.

The influence of actin depolymerization induced by Cytochalasin D and mechanical stretch on interleukin-8 expression and JNK phosphorylation levels in human retinal pigment epithelial cells
Gao M, Wu S, Ji J, Zhang J, Liu Q, Yue Y, Liu L, Liu X, Liu W. BMC Ophthalmol 17(1):43, 2017. doi: 10.1186/s12886-017-0437-z.

Orthodontic strain affects the Hippo-pathway effector YAP concomitant with proliferation in human periodontal ligament fibroblasts
Huelter-Hassler D, Tomakidi P, Steinberg T, Jung BA. Eur J Orthod 2017 Mar 17. doi: 10.1093/ejo/cjx012. [Epub ahead of print]

Periodontal ligament stem cells in the periodontitis microenvironment are sensitive to static mechanical strain
Liu J, Li Q, Liu S, Gao J, Qin W, Song Y, Jin Z. Stem Cells Int 2017:1380851, 2017. doi: 10.1155/2017/1380851.

Rhomboid family member 2 regulates cytoskeletal stress-associated Keratin 16
Maruthappu T, Chikh A, Fell B, Delaney PJ, Brooke MA, Levet C, Moncada-Pazos A, Ishida-Yamamoto A, Blaydon D, Waseem A, Leigh IM, Freeman M, Kelsell DP. Nat Commun 8:14174, 2017. doi: 10.1038/ncomms14174.

Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress
Ning S, Chen Z, Fan D, Sun C, Zhang C, Zeng Y, Li W, Hou X, Qu X, Ma Y, Yu H. Int J Mol Med 39(1):135-143, 2017. doi: 10.3892/ijmm.2016.2803.

VEGF as a paracrine regulator of conventional outflow facility
Reina-Torres E, Wen JC, Liu KC, Li G, Sherwood JM, Chang JY, Challa P, Flügel-Koch CM, Stamer WD, Allingham RR, Overby DR. Invest Ophthalmol Vis Sci 58(3):1899-1908, 2017. doi: 10.1167/iovs.16-20779.

Evidence of the role of R-spondin 1 and its receptor Lgr4 in the transmission of mechanical stimuli to biological signals for bone formation
Shi GX, Zheng XF, Zhu C, Li B, Wang YR, Jiang SD, Jiang LS. Int J Mol Sci 18(3), 2017. pii: E564. doi: 10.3390/ijms18030564.

Mechanical loading improves tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model by promoting proliferation and matrix formation of mesenchymal stem cells and tendon cells
Song F, Jiang D, Wang T, Wang Y, Chen F, Xu G, Kang Y, Zhang Y. Cell Physiol Biochem 41(3):875-889, 2017. doi: 10.1159/000460005.

Bioengineering approaches to mature human pluripotent stem cell-derived cardiomyocytes
Sun X, Nunes SS. Front Cell Dev Biol 5:19, 2017. doi: 10.3389/fcell.2017.00019.

The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse
Wang JC, Lee JY, Christian S, Dang-Lawson M, Pritchard C, Freeman SA, Gold MR. J Cell Sci 130(6):1094-1109, 2017. doi: 10.1242/jcs.191858.

Secreted miR-27a induced by cyclic stretch modulates the proliferation of endothelial cells in hypertension via GRK6
Wang L, Bao H, Wang KX, Zhang P, Yao QP, Chen XH, Huang K, Qi YX, Jiang ZL. Sci Rep 7:41058, 2017. doi: 10.1038/srep41058.

Effect of mechanical stretch on apoptosis and Bax/Bcl-2 protein expression of human oral squamous cell carcinoma KB cells in vitro
Wang Y, Qu H. In: Proceedings of the 2016 International Conference on Biotechnology & Medical Science. Ed. Y Zhang. World Scientific Publishing Co. Pte. Ltd.: Singapore, 191-198, 2017.

Profiles of long noncoding RNAs in hypertensive rats: long noncoding RNA XR007793 regulates cyclic strain-induced proliferation and migration of vascular smooth muscle cells
Yao QP, Xie ZW, Wang KX, Zhang P, Han Y, Qi YX, Jiang ZL. J Hypertens 2017 Mar 17. doi: 10.1097/HJH.0000000000001304. [Epub ahead of print]

Effects of transient receptor potential canonical 1 (TRPC1) on the mechanical stretch-induced expression of airway remodeling-associated factors in human bronchial epithelioid cells
Yu Q, Li M. J Biomech 51:89-96, 2017. doi: 10.1016/j.jbiomech.2016.12.002.

Mechanical injury induces brain endothelial-derived microvesicle release: implications for cerebral vascular injury during traumatic brain injury
Andrews AM, Lutton EM, Merkel SF, Razmpour R, Ramirez SH. Front Cell Neurosci 10:43, 2016. doi: 10.3389/fncel.2016.00043.

Sonic hedgehog stimulates neurite outgrowth in a mechanical stretch model of reactive-astrogliosis
Berretta A, Gowing EK, Jasoni CL, Clarkson AN. Sci Rep 6:21896, 2016. doi: 10.1038/srep21896.

MP89-20 Traction applied to Peyronie's disease cells reduces cellular fibroisis
Campbell J, DeYoung L, Chung E, Brock G. J Urol 195 (4):e1144, 2016.

Connexin 43 promotes ossification of the posterior longitudinal ligament through activation of the ERK1/2 and p38 MAPK pathways
Chen D, Liu Y, Yang H, Chen D, Zhang X, Fermandes JC, Chen Y. Cell Tissue Res 363(3):765-73, 2016. doi: 10.1007/s00441-015-2277-6.

Cyclic equibiaxial tensile strain alters gene expression of chondrocytes via histone deacetylase 4 shuttling
Chen C, Wei X, Lv Z, Sun X, Wang S, Zhang Y, Jiao Q, Wang X, Li Y, Wei L. PLoS One 11(5):e0154951, 2016. doi: 10.1371/journal.pone.0154951.

Mechanical stretch inhibits microRNA499 via p53 to regulate calcineurin-A expression in rat cardiomyocytes
Chua SK, Wang BW, Lien LM, Lo HM, Chiu CZ, Shyu KG. PLoS One 11(2):e0148683, 2016. doi: 10.1371/journal.pone.0148683.

Varicose remodeling of veins is suppressed by 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors
Eschrich J, Meyer R, Kuk H, Wagner AH, Noppeney T, Debus S, Hecker M, Korff T. J Am Heart Assoc 5(2), 2016. pii: e002405. doi: 10.1161/JAHA.115.002405.

p38α MAPK inhibits stretch-induced JNK activation in cardiac myocytes through MKP-1
Feng H, Gerilechaogetu F, Golden HB, Nizamutdinov D, Foster DM, Glaser SS, Dostal DE. Int J Cardiol 203:145-55, 2016. doi: 10.1016/j.ijcard.2015.10.109.

Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
Feng P, Li X, Chen W, Liu C, Rong S, Wang X, Du G. Biomed Eng Online 15(1):63, 2016. doi: 10.1186/s12938-016-0198-6.

Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload
Fu L, Wei CC, Powell PC, Bradley WE, Ahmad S, Ferrario CM, Collawn JF, Dell'Italia LJ. J Mol Cell Cardiol 92:1-9, 2016. doi: 10.1016/j.yjmcc.2016.01.019.

Cyclic stretch promotes osteogenesis-related gene expression in osteoblast-like cells through a cofilin-associated mechanism
Gao J, Fu S, Zeng Z, Li F, Niu Q, Jing D, Feng X. Mol Med Rep 14(1):218-24, 2016. doi: 10.3892/mmr.2016.5239. Epub 2016 May 10.

Impacts of dynamic mechanical stretch on the expression of plasminogen activator inhibitor-1 (PAI-1) in human A549 cell
Guo Y, Zhang W, Zheng L, Guo W, Zhang H, Li X. Int J Clin Exp Pathol 9(6):5871-5881, 2016.

Hypercapnic acidosis attenuates pulmonary epithelial stretch-induced injury via inhibition of the canonical NF-κB pathway
Horie S, Ansari B, Masterson C, Devaney J, Scully M, O'Toole D, Laffey JG. Intensive Care Med Exp 4(1):8, 2016. doi: 10.1186/s40635-016-0081-6.

Gtf2ird1-Dependent mohawk expression regulates mechanosensing properties of the tendon
Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H. Mol Cell Biol 36(8):1297-309, 2016. doi: 10.1128/MCB.00950-15.

Dynamic mechanical and nanofibrous topological combinatory cues designed for periodontal ligament engineering
Kim JH, Kang MS, Eltohamy M, Kim TH, Kim HW. PLoS One 11(3):e0149967, 2016. doi: 10.1371/journal.pone.0149967.

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip.
Kim HJ, Li H, Collins JJ, Ingber DE. Proc Natl Acad Sci U S A 113(1):E7-15, 2016. doi: 10.1073/pnas.1522193112.

Mechanical stretching stimulates collagen synthesis via down-regulating SO2/AAT1 pathway
Liu J, Yu W, Liu Y, Chen S, Huang Y, Li X, Liu C, Zhang Y, Li Z, Du J, Tang C, Du J, Jin H. Sci Rep 6:21112, 2016. doi: 10.1038/srep21112.

Mechanical strain causes adaptive change in bronchial fibroblasts enhancing profibrotic and inflammatory responses
Manuyakorn W, Smart DE, Noto A, Bucchieri F, Haitchi HM, Holgate ST, Howarth PH, Davies DE. PLoS One 11(4):e0153926, 2016. doi: 10.1371/journal.pone.0153926.

Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application
Qi YX, Yao QP, Huang K, Shi Q, Zhang P, Wang GL, Han Y, Bao H, Wang L, Li HP, Shen BR, Wang Y, Chien S, Jiang ZL. Proc Natl Acad Sci U S A 113(19):5293-8, 2016. doi: 10.1073/pnas.1604569113.

Context-dependent switch in chemo/mechanotransduction via multilevel crosstalk among cytoskeleton-regulated MRTF and TAZ and TGFβ-regulated Smad3
Speight P, Kofler M, Szászi K, Kapus A. Nat Commun 7:11642, 2016. doi: 10.1038/ncomms11642

Effects of mechanical stretch on cell proliferation and matrix formation of mesenchymal stem cell and anterior cruciate ligament fibroblast
Sun L, Qu L, Zhu R, Li H, Xue Y, Liu X, Fan J, Fan H. Stem Cells International 2016(9842075), 10 pages, 2016. doi:10.1155/2016/9842075.

Modulation of endothelial inflammation by low and high magnitude cyclic stretch
Tian Y, Gawlak G, O'Donnell JJ 3rd, Mambetsariev I, Birukova AA. PLoS One 11(4):e0153387, 2016. doi: 10.1371/journal.pone.0153387.

NF-κB signaling pathway in controlling intervertebral disk cell response to inflammatory and mechanical stressors
Tisherman R, Coelho P, Phillibert D, Wang D, Dong Q, Vo N, Kang J, Sowa G. Phys Ther 2016 Feb 18. [Epub ahead of print].

Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells
Trumbull A, Subramanian G, Yildirim-Ayan E. Biomed Eng Online 15:43, 2016. doi: 10.1186/s12938-016-0150-9.

Mechanical stimulation orchestrates the osteogenic differentiation of human bone marrow stromal cells by regulating HDAC1
Wang J, Wang CD, Zhang N, Tong WX, Zhang YF, Shan SZ, Zhang XL, Li QF. Cell Death Dis 7:e2221, 2016. doi: 10.1038/cddis.2016.112.

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk
Xu HG, Zheng Q, Song JX, Li J, Wang H, Liu P, Wang J, Wang CD, Zhang XL. Osteoarthritis Cartilage 24(1):158-68, 2016. doi: 10.1016/j.joca.2015.07.019.

Engineering tendon: scaffolds, bioreactors, and models of regeneration
Youngstrom DW, Barrett JG. Stem Cells Int 2016:3919030, 2016. doi: 10.1155/2016/3919030.

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