Cell Seeder

Flexcell® HT Cell Seeders™
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Provides a mold for users to plate cells in the central region of the well of a 24-well HT BioFlex® plate during seeding (see Fig. 1 below).
  • Helps to prevent cells from being subjected to undefined strains when using equibiaxial Loading Stations™.
  • Available in a set of four or as part of a baseplate kit.
Read more about Applying Mechanical Load to Cells in Monolayer Culture

Cell Seeder

Figure 1: Well of a 24-well HT BioFlex® plate with the rubber membrane pulled into a HT Cell Seeder™ sitting beneath the well (Left). Cells stained with crystal violet three days after seeding at 30,000 cells/cm2 (Right).

Relevant Tech Reports & Other Information
Recent Publications with a Flexcell® Tension Product

Mechanical injury induces brain endothelial-derived microvesicle release: implications for cerebral vascular injury during traumatic brain injury
Andrews AM, Lutton EM, Merkel SF, Razmpour R, Ramirez SH. Front Cell Neurosci 10:43, 2016. doi: 10.3389/fncel.2016.00043.

Sonic hedgehog stimulates neurite outgrowth in a mechanical stretch model of reactive-astrogliosis
Berretta A, Gowing EK, Jasoni CL, Clarkson AN. Sci Rep 6:21896, 2016. doi: 10.1038/srep21896.

MP89-20 Traction applied to Peyronie's disease cells reduces cellular fibroisis
Campbell J, DeYoung L, Chung E, Brock G. J Urol 195 (4):e1144, 2016.

Connexin 43 promotes ossification of the posterior longitudinal ligament through activation of the ERK1/2 and p38 MAPK pathways
Chen D, Liu Y, Yang H, Chen D, Zhang X, Fermandes JC, Chen Y. Cell Tissue Res 363(3):765-73, 2016. doi: 10.1007/s00441-015-2277-6. Epub 2015 Sep 3.

Cyclic equibiaxial tensile strain alters gene expression of chondrocytes via histone deacetylase 4 shuttling
Chen C, Wei X, Lv Z, Sun X, Wang S, Zhang Y, Jiao Q, Wang X, Li Y, Wei L. PLoS One 11(5):e0154951, 2016. doi: 10.1371/journal.pone.0154951.

Mechanical stretch inhibits microRNA499 via p53 to regulate calcineurin-A expression in rat cardiomyocytes
Chua SK, Wang BW, Lien LM, Lo HM, Chiu CZ, Shyu KG. PLoS One 11(2):e0148683, 2016. doi: 10.1371/journal.pone.0148683.

Varicose remodeling of veins is suppressed by 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors
Eschrich J, Meyer R, Kuk H, Wagner AH, Noppeney T, Debus S, Hecker M, Korff T. J Am Heart Assoc 5(2), 2016. pii: e002405. doi: 10.1161/JAHA.115.002405.

p38α MAPK inhibits stretch-induced JNK activation in cardiac myocytes through MKP-1
Feng H, Gerilechaogetu F, Golden HB, Nizamutdinov D, Foster DM, Glaser SS, Dostal DE. Int J Cardiol 203:145-55, 2016. doi: 10.1016/j.ijcard.2015.10.109. Epub 2015 Oct 23.

Combined effects of interleukin-1β and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro
Feng P, Li X, Chen W, Liu C, Rong S, Wang X, Du G. Biomed Eng Online 15(1):63, 2016. doi: 10.1186/s12938-016-0198-6.

Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload
Fu L, Wei CC, Powell PC, Bradley WE, Ahmad S, Ferrario CM, Collawn JF, Dell'Italia LJ. J Mol Cell Cardiol 92:1-9, 2016. doi: 10.1016/j.yjmcc.2016.01.019. Epub 2016 Jan 22.

Cyclic stretch promotes osteogenesis-related gene expression in osteoblast-like cells through a cofilin-associated mechanism
Gao J, Fu S, Zeng Z, Li F, Niu Q, Jing D, Feng X. Mol Med Rep 14(1):218-24, 2016. doi: 10.3892/mmr.2016.5239. Epub 2016 May 10.

Impacts of dynamic mechanical stretch on the expression of plasminogen activator inhibitor-1 (PAI-1) in human A549 cell
Guo Y, Zhang W, Zheng L, Guo W, Zhang H, Li X. Int J Clin Exp Pathol 9(6):5871-5881, 2016.

Hypercapnic acidosis attenuates pulmonary epithelial stretch-induced injury via inhibition of the canonical NF-κB pathway
Horie S, Ansari B, Masterson C, Devaney J, Scully M, O'Toole D, Laffey JG. Intensive Care Med Exp 4(1):8, 2016. doi: 10.1186/s40635-016-0081-6.

Gtf2ird1-Dependent mohawk expression regulates mechanosensing properties of the tendon
Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H. Mol Cell Biol 36(8):1297-309, 2016. doi: 10.1128/MCB.00950-15.

Dynamic mechanical and nanofibrous topological combinatory cues designed for periodontal ligament engineering
Kim JH, Kang MS, Eltohamy M, Kim TH, Kim HW. PLoS One 11(3):e0149967, 2016. doi: 10.1371/journal.pone.0149967.

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip.
Kim HJ, Li H, Collins JJ, Ingber DE. Proc Natl Acad Sci U S A 113(1):E7-15, 2016. doi: 10.1073/pnas.1522193112. Epub 2015 Dec 14.

Mechanical stretching stimulates collagen synthesis via down-regulating SO2/AAT1 pathway
Liu J, Yu W, Liu Y, Chen S, Huang Y, Li X, Liu C, Zhang Y, Li Z, Du J, Tang C, Du J, Jin H. Sci Rep 6:21112, 2016. doi: 10.1038/srep21112.

Mechanical strain causes adaptive change in bronchial fibroblasts enhancing profibrotic and inflammatory responses
Manuyakorn W, Smart DE, Noto A, Bucchieri F, Haitchi HM, Holgate ST, Howarth PH, Davies DE. PLoS One 11(4):e0153926, 2016. doi: 10.1371/journal.pone.0153926.

Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application
Qi YX, Yao QP, Huang K, Shi Q, Zhang P, Wang GL, Han Y, Bao H, Wang L, Li HP, Shen BR, Wang Y, Chien S, Jiang ZL. Proc Natl Acad Sci U S A 113(19):5293-8, 2016. doi: 10.1073/pnas.1604569113. Epub 2016 Apr 25.

Context-dependent switch in chemo/mechanotransduction via multilevel crosstalk among cytoskeleton-regulated MRTF and TAZ and TGFβ-regulated Smad3
Speight P, Kofler M, Szászi K, Kapus A. Nat Commun 7:11642, 2016. doi: 10.1038/ncomms11642

Effects of mechanical stretch on cell proliferation and matrix formation of mesenchymal stem cell and anterior cruciate ligament fibroblast
Sun L, Qu L, Zhu R, Li H, Xue Y, Liu X, Fan J, Fan H. Stem Cells International 2016(9842075), 10 pages, 2016. doi:10.1155/2016/9842075.

Modulation of endothelial inflammation by low and high magnitude cyclic stretch
Tian Y, Gawlak G, O'Donnell JJ 3rd, Mambetsariev I, Birukova AA. PLoS One 11(4):e0153387, 2016. doi: 10.1371/journal.pone.0153387.

NF-κB signaling pathway in controlling intervertebral disk cell response to inflammatory and mechanical stressors
Tisherman R, Coelho P, Phillibert D, Wang D, Dong Q, Vo N, Kang J, Sowa G. Phys Ther 2016 Feb 18. [Epub ahead of print].

Mechanoresponsive musculoskeletal tissue differentiation of adipose-derived stem cells
Trumbull A, Subramanian G, Yildirim-Ayan E. Biomed Eng Online 15:43, 2016. doi: 10.1186/s12938-016-0150-9.

Mechanical stimulation orchestrates the osteogenic differentiation of human bone marrow stromal cells by regulating HDAC1
Wang J, Wang CD, Zhang N, Tong WX, Zhang YF, Shan SZ, Zhang XL, Li QF. Cell Death Dis 7:e2221, 2016. doi: 10.1038/cddis.2016.112.

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk
Xu HG, Zheng Q, Song JX, Li J, Wang H, Liu P, Wang J, Wang CD, Zhang XL. Osteoarthritis Cartilage 24(1):158-68, 2016. doi: 10.1016/j.joca.2015.07.019. Epub 2015 Aug 3.

Engineering tendon: scaffolds, bioreactors, and models of regeneration
Youngstrom DW, Barrett JG. Stem Cells Int 2016:3919030, 2016. doi: 10.1155/2016/3919030. Epub 2015 Dec 28.

Improving bone formation in osteoporosis through in vitro mechanical stimulation compared to biochemical stimuli
Acosta FL, Pham M, Safai Y, Buser Z. Journal of Nature and Science 1(4):e63, 2015.

Modeling the impact of scaffold architecture and mechanical loading on collagen turnover in engineered cardiovascular tissues
Argento G, de Jonge N, Söntjens SH, Oomens CW, Bouten CV, Baaijens FP. Biomech Model Mechanobiol 14(3):603-13, 2015. doi: 10.1007/s10237-014-0625-1.

Effects of cyclic tensile strain on chondrocyte metabolism: a systematic review
Bleuel J, Zaucke F, Brüggemann GP, Niehoff A. PLoS One 10(3):e0119816, 2015. doi: 10.1371/journal.pone.0119816.

Mechanical stretch induces apoptosis regulator TRB3 in cultured cardiomyocytes and volume-overloaded heart
Cheng WP, Wang BW, Lo HM, Shyu KG. PLoS One 10(4):e0123235, 2015. doi: 10.1371/journal.pone.0123235.

Investigation of human embryonic stem cell-derived keratinocytes as an in vitro research model for mechanical stress dynamic response
Cherbuin T, Movahednia MM, Toh WS, Cao T. Stem Cell Rev 11(3):460-73, 2015. doi: 10.1007/s12015-014-9565-5.

Three-dimensional development of tensile pre-strained annulus fibrosus cells for tissue regeneration: An in-vitro study
Chuah YJ, Lee WC, Wong HK, Kang Y, Hee HT. Exp Cell Res 331(1):176-82, 2015. doi: 10.1016/j.yexcr.2014.09.022.

Preclinical models for in vitro mechanical loading of bone-derived cells
Delaine-Smith RM, Javaheri B, Helen Edwards J, Vazquez M, Rumney RM. Bonekey Rep 4:728, 2015. doi: 10.1038/bonekey.2015.97.

Blockade of the acute activation of mTOR complex 1 decreases hypertrophy development in rats with severe aortic valve regurgitation
Drolet MC, Desbiens-Brassard V, Roussel E, Tu V, Couet J, Arsenault M. Springerplus 4:435, 2015. doi: 10.1186/s40064-015-1230-1.

Loss of the mechanotransducer zyxin promotes a synthetic phenotype of vascular smooth muscle cells
Ghosh S, Kollar B, Nahar T, Suresh Babu S, Wojtowicz A, Sticht C, Gretz N, Wagner AH, Korff T, Hecker M. J Am Heart Assoc 4(6):e001712, 2015. doi: 10.1161/JAHA.114.001712.

Cell cycle- and cancer-associated gene networks activated by Dsg2: evidence of cystatin a deregulation and a potential role in cell-cell adhesion
Gupta A, Nitoiu D, Brennan-Crispi D, Addya S, Riobo NA, Kelsell DP, Mahoney MG. PLoS One 10(3):e0120091, 2015. doi: 10.1371/journal.pone.0120091.

Cyclic strain enhances cellular uptake of nanoparticles
Hu J, Liu Y. Journal of Nanomaterials 2015:953584, 2015. doi:10.1155/2015/953584.

Cyclic stretch augments production of neutrophil chemokines, matrix metalloproteinases, and activin A in human endometrial stromal cells
Izumi G, Koga K, Nagai M, Urata Y, Takamura M, Harada M, Hirata T, Hirota Y, Ogawa K, Inoue S, Fujii T, Osuga Y. Am J Reprod Immunol 2015 Jan 21. doi: 10.1111/aji.12359.

Mechanical stretch-induced endoplasmic reticulum stress, apoptosis and inflammation contribute to thoracic aortic aneurysm and dissection
Jia LX, Zhang WM, Zhang HJ, Li TT, Wang YL, Qin YW, Gu H, Du J. J Pathol 236(3):373-83, 2015. doi: 10.1002/path.4534.

Tension force downregulates matrix metalloproteinase expression and upregulates the expression of their inhibitors through MAPK signaling pathways in MC3T3-E1 cells.
Karasawa Y, Tanaka H, Nakai K, Tanabe N, Kawato T, Maeno M, Shimizu N. Int J Med Sci 12(11):905-13, 2015. doi: 10.7150/ijms.13263.

Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts
Kariya T, Tanabe N, Shionome C, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N. J Cell Biochem 116(1):12-21, 2015. doi: 10.1002/jcb.24863.

CytoSpectre: a tool for spectral analysis of oriented structures on cellular and subcellular levels
Kartasalo K, Pölönen RP, Ojala M, Rasku J, Lekkala J, Aalto-Setälä K, Kallio P. BMC Bioinformatics 16:344, 2015. doi: 10.1186/s12859-015-0782-y.

Influence of mechanical stimulation on human dermal fibroblasts derived from different body sites
Kuang R, Wang Z, Xu Q, Liu S, Zhang W. Int J Clin Exp Med 8(5):7641-7, 2015.

Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment
Le HQ, Ghatak S, Yeung CY, Tellkamp F, Günschmann C, Dieterich C, Yeroslaviz A, Habermann B, Pombo A, Niessen CM, Wickström SA. Nat Cell Biol 18(8):864-75, 2016. doi: 10.1038/ncb3387. Epub 2016 Jul 11.

Surface modification of uniaxial cyclic strain cell culture platform with temperature-responsive polymer for cell sheet detachment
Lee EL, Bendre HH, Kalmykov A, Wong JY. J Mater Chem B Mater Biol Med 3(40):7899-7902, 2015. Epub 2015 Aug 19.

Mechanical stretch induces angiotensinogen expression through PARP1 activation in kidney proximal tubular cells
Lee JS, Lim JY, Kim J. In Vitro Cell Dev Biol Anim 51(1):72-8, 2015. doi: 10.1007/s11626-014-9809-3.

Cyclic mechanical strain of myocytes modifies CapZβ1 post translationally via PKCε
Lin YH, Swanson ER, Li J, Mkrtschjan MA, Russell B. J Muscle Res Cell Motil 36(4-5):329-37, 2015. doi: 10.1007/s10974-015-9420-6. Epub 2015 Oct 1.

Loss-of-function mutations in CAST cause peeling skin, leukonychia, acral punctate keratoses, cheilitis, and knuckle pads
Lin Z, Zhao J, Nitoiu D, Scott CA, Plagnol V, Smith FJ, Wilson NJ, Cole C, Schwartz ME, McLean WH, Wang H, Feng C, Duo L, Zhou EY, Ren Y, Dai L, Chen Y, Zhang J, Xu X, O'Toole EA, Kelsell DP, Yang Y. Am J Hum Genet 96(3):440-7, 2015. doi: 10.1016/j.ajhg.2014.12.026.

Effects of physiologic mechanical stimulation on embryonic chick cardiomyocytes using a microfluidic cardiac cell culture model
Nguyen MD, Tinney JP, Ye F, Elnakib AA, Yuan F, El-Baz A, Sethu P, Keller BB, Giridharan GA. Anal Chem 87(4):2107-13, 2015. doi: 10.1021/ac503716z. Epub 2015 Feb 2.

TNFα shedding in mechanically stressed cardiomyocytes is mediated by Src activation of TACE
Niu A, Wang B, Li YP. J Cell Biochem 116(4):559-65, 2015. doi: 10.1002/jcb.25006.

Dyssynchronous pacing triggers endothelial-mesenchymal transition through heterogeneity of mechanical stretch in a canine model
Mai J, Hu Q, Xie Y, Su S, Qiu Q, Yuan W, Yang Y, Song E, Chen Y, Wang J. Circ J 79(1):201-9, 2015. doi: 10.1253/circj.CJ-14-0721.

Variability in vascular smooth muscle cell stretch-induced responses in 2D culture
Mantella LE, Quan A, Verma S. Vasc Cell 7:7, 2015. doi: 10.1186/s13221-015-0032-0.

Arterial expression of the calcium-sensing receptor is maintained by physiological pulsation and protects against calcification
Molostvov G, Hiemstra TF, Fletcher S, Bland R, Zehnder D. PLoS One 10(10):e0138833, 2015. doi: 10.1371/journal.pone.0138833.

The stretch responsive microRNA miR-148a-3p is a novel repressor of IKBKB, NF-κB signaling, and inflammatory gene expression in human aortic valve cells
Patel V, Carrion K, Hollands A, Hinton A, Gallegos T, Dyo J, Sasik R, Leire E, Hardiman G, Mohamed SA, Nigam S, King CC, Nizet V, Nigam V. FASEB J 29(5):1859-68, 2015. doi: 10.1096/fj.14-257808.

Polycystin-1 is a cardiomyocyte mechanosensor that governs L-type Ca2+ channel protein stability
Pedrozo Z, Criollo A, Battiprolu PK, Morales CR, Contreras-Ferrat A, Fernández C, Jiang N, Luo X, Caplan MJ, Somlo S, Rothermel BA, Gillette TG, Lavandero S, Hill JA. Circulation 131(24):2131-42, 2015. doi: 10.1161/CIRCULATIONAHA.114.013537. Epub 2015 Apr 17.

Cyclic mechanical strain induces TGFβ1-signalling in dermal fibroblasts embedded in a 3D collagen lattice
Peters AS, Brunner G, Krieg T, Eckes B. Arch Dermatol Res 307(2):191-7, 2015. doi: 10.1007/s00403-014-1514-2.

Simultaneous increases in proliferation and apoptosis of vascular smooth muscle cells accelerate diabetic mouse venous atherosclerosis
Ping S, Li Y, Liu S, Zhang Z, Wang J, Zhou Y, Liu K, Huang J, Chen D, Wang J, Li C. PLoS One 10(10):e0141375, 2015. doi: 10.1371/journal.pone.0141375.

High-magnitude and/or high-frequency mechanical strain promotes peripapillary scleral myofibroblast differentiation
Qu J, Chen H, Zhu L, Ambalavanan N, Girkin CA, Murphy-Ullrich JE, Downs JC, Zhou Y. Invest Ophthalmol Vis Sci 56(13):7821-30, 2015. doi: 10.1167/iovs.15-17848.

Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes
Raval KK, Tao R, White BE, De Lange WJ, Koonce CH, Yu J, Kishnani PS, Thomson JA, Mosher DF, Ralphe JC, Kamp TJ. J Biol Chem 290(5):3121-36, 2015. doi: 10.1074/jbc.M114.628628.

Effects of intermittent and incremental cyclic stretch on ERK signaling and collagen production in engineered tissue
Schmidt JB, Chen K, Tranquillo RT. Cellular and Molecular Bioengineering 1-10, 2015. doi:10.1007/s12195-015-0415-6.

Competition between cap and basal actin fiber orientation in cells subjected to contact guidance and cyclic strain
Tamiello C, Bouten CV, Baaijens FP. Sci Rep 5:8752, 2015. doi: 10.1038/srep08752.

Expression of heat shock protein 27 correlates with actin cytoskeletal dynamics and contractility of cultured human bladder smooth muscle cells
Wang Y, Xiong Z, Gong W, Zhou P, Xie Q, Zhou Z, Lu G. Exp Cell Res 338(1):39-44, 2015. doi: 10.1016/j.yexcr.2015.08.002.

Hypoxia inducible factor signaling and experimental persistent pulmonary hypertension of the newborn
Wedgwood S, Lakshminrusimha S, Schumacker PT, Steinhorn RH. Front Pharmacol 6:47, 2015. doi: 10.3389/fphar.2015.00047.

Galectin-3 level and the severity of cardiac diastolic dysfunction using cellular and animal models and clinical indices
Wu CK, Su MY, Lee JK, Chiang FT, Hwang JJ, Lin JL, Chen JJ, Liu FT, Tsai CT. Sci Rep 5:17007, 2015. doi: 10.1038/srep17007.

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk
Xu HG, Zheng Q, Song JX, Li J, Wang H, Liu P, Wang J, Wang CD, Zhang XL. Osteoarthritis Cartilage 24(1):158-68, 2016. doi: 10.1016/j.joca.2015.07.019. Epub 2015 Aug 3.

Modeled osteopathic manipulative treatments: A review of their in vitro effects on fibroblast tissue preparations
Zein-Hammoud M, Standley PR. J Am Osteopath Assoc 115(8):490-502, 2015. doi: 10.7556/jaoa.2015.103.

Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an mTOR signaling-associated mechanism
Zeng Z, Jing D, Zhang X, Duan Y, Xue F. Int J Mol Med 36(4):947-56, 2015. doi: 10.3892/ijmm.2015.2304. Epub 2015 Aug 5.

Cyclic stretch enhances bone morphogenetic protein-2-induced osteoblastic differentiation through the inhibition of Hey1
Zeng Z, Yin X, Zhang X, Jing D, Feng X. Int J Mol Med 36(5):1273-1281, 2015. doi: 10.3892/ijmm.2015.2354. Epub 2015 Sep 24.

Identification of biomechanical force as a novel inducer of epithelial-mesenchymal transition features in mechanical stretched skin
Zhou J, Wang J, Zhang N, Zhang Y, Li Q. Am J Transl Res 7(11):2187-2198, 2015.

microRNA-103a functions as a mechanosensitive microRNA to inhibit bone formation through targeting Runx2
Zuo B, Zhu J, Li J, Wang C, Zhao X, Cai G, Li Z, Peng J, Wang P, Shen C, Huang Y, Xu J, Zhang X, Chen X. J Bone Miner Res 30(2):330-45, 2015. doi: 10.1002/jbmr.2352.

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