Arctangle® Loading Stations™ 6-well

Uniaxial strain is achieved through selectively controlling the portion of the flexible membrane that is exposed to regulated vacuum. This is achieved through the use of a specially designed Arctangle® Loading Station™ (Fig. 1), which is comprised of a3.3” x 5” Lexan® plate, two nylon support centering posts, and six removable nylon planar faced cylinders, or loading posts. The two centering posts are intended to support the UniFlex® or TissueTrain® plate under high vacuum and also center the plate over the six loading posts. The six loading posts provide the strain surface.The posts are positioned on the Lexan® plate such that each is centered beneath the 35 mm well bottom of a UniFlex® or TissueTrain® culture plate (Fig. 1C). When vacuum is applied to a culture plate with a Flexcell® Tension System, the membrane deforms across the loading post face only at the east and west poles creating uniaxial strain.

Arctangle® Loading Stations™ have maximum and minimum strain capabilities with respect to vacuum level. Table 1 lists the minimum and maximum % elongations for both the UniFlex® and TissueTrain® culture plates when used with the Arctangle® LoadingStations™ and the FX-5000™ Tension System. When creating regimens, do not exceed these values in the min% and max% boxes for experiments in which the Arctangle® Loading Station™ will be used.

A) Arctangle® loading post. B) UniFlex® 6-well plate. C) UniFlex® culture plate resting atop Arctangle® Loading Station™.

Supporting Publications

At Flexcell® International Corporation, we understand that our commitment to the highest quality products and customer service depend on our ability to take part in research and development continuously. We invite you to read more about our ongoing research and development on cell stretching bioreactors in tissue engineering, cellular mechanics, gene and protein expression, cytomechanics, drug discovery, orthopedics, cardiovascular, and pulmonary research.

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