Cells sense soft!

CellSoft® is available in 100 mm diameter round dishes, pre-coated in a specially formulated silicone elastomer soft substrate. Moduli stiffness ranges from 1.0 - 60 kPa. CellSoft® Soft Substrate culture dishes come Untreated or with covalently bonded Collagen (Type I) protein matrix to improve cell attachment. Flexcell® recommends that users culture their stock cells on a given stiffness substrate with a matrix coating that have the same surface parameters when conducting their experiment. This way, the cells do not experience “substrate shock” moving from one stiffness and matrix coating to another. Users can repeatedly trypsinize and replate cells on the CellSoft®, matrix-coated surfaces up to three times.